Transcriptomic Analysis of Permafrost Mucormycete Stress on Fennel Thin-Winged Borer

Abstract

In this study, a comprehensive transcriptome analysis was performed on Evergestis extimalis Scoli under the stress of Mucor permafrost by transcriptome sequencing technology, aiming to reveal the regulatory mechanism and biological characteristics of gene expression under pathogenic fungal stress. The experiment used high-throughput sequencing technology to annotate Unigenes using multiple databases, and found that Asian corn borer was the species with the most homologous sequences. A total of 357, 444, 198 and 890 differentially expressed genes were detected by differential expression analysis, which were mainly enriched in cell components, molecular functions and biological processes, involving polysaccharide degradation, metabolic processes, and antibacterial humoral reaction regulation. KEGG enrichment analysis revealed multiple pathways related to insect metabolism and signaling, such as "oxidative phosphorylation", "glycolysis/gluconeogenesis" and "MAPK signaling pathway", among which the environmental information processing-signal transduction pathway enriched the most differential genes. In addition, real-time PCR verified that the expression trends of 9 genes with large differences were basically consistent with the transcriptome sequencing results, indicating that the transcriptome data had high accuracy and reliability. The results of this study provide an important theoretical basis for understanding the gene expression regulation mechanism and biological characteristics of F. fennel under permafrost Mucormycete stress.